Journal: Oncotarget

Article Title: Connexin 32 dysfunction promotes ethanol-related hepatocarcinogenesis via activation of Dusp1-Erk axis

doi:

Figure Lengend Snippet: Up-regulated and down-regulated genes in the liver of EtOH-treated Tg rats

Article Snippet: Thereafter a polyclonal rabbit antibody against GST-P was used with biotin-conjugated anti-rabbit IgG and Cy5-labeled streptavidin (Thermo Fischer Scientific Inc.), and finally the section was incubated with an Alexa Fluor 350 conjugated rabbit anti-DUSP1 polyclonal antibody (Bioss Inc., Boston, MA).

Techniques:

Journal: Oncotarget

Article Title: Connexin 32 dysfunction promotes ethanol-related hepatocarcinogenesis via activation of Dusp1-Erk axis

doi:

Figure Lengend Snippet: Levels of Dusp1 mRNA A. and protein B. in whole liver tissues measured by quantitative RT-PCR and western blotting, respectively. Data of quantitative RT-PCR are presented as mean ± SD, n = 4 per groups. **, ***: P < 0.01 and 0.001 respectively. Representative image of laser microdissected tissue (upper) and Dusp1 mRNA level in GST-P positive foci as determined by quantitative RT-PCR (lower) C. Immunohistochemical staining for Dusp1 in GST-P positive foci of Tg and Wt rats that received 5%EtOH (upper) and the intensity score of Dusp1 in GST-P positive foci as compared to Wt-control rats (lower) D. Data are presented as mean ± SD, n = 5 per group, 10 foci/rat. *: P < 0.05.

Article Snippet: Thereafter a polyclonal rabbit antibody against GST-P was used with biotin-conjugated anti-rabbit IgG and Cy5-labeled streptavidin (Thermo Fischer Scientific Inc.), and finally the section was incubated with an Alexa Fluor 350 conjugated rabbit anti-DUSP1 polyclonal antibody (Bioss Inc., Boston, MA).

Techniques: Quantitative RT-PCR, Western Blot, Immunohistochemical staining, Staining

Journal: BMC Veterinary Research

Article Title: Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells

doi: 10.1186/s12917-023-03757-3

Figure Lengend Snippet: Immunocytochemistry of neural stem/progenitor cells (NSPCs) cultured as neurospheres or monolayers. (A) 3rd, 6th, and 9th passage cells cultured in a monolayer for 5 DIV. (B) 3rd, 6th, and 9th passage cells cultured as neurosphere for 5 DIV. Nuclei were stained with DAPI (blue) and NSPCs marker for SOX2 (green) and nestin (red). (C) Each well of the 24-well plate was immunostained with nestin and SOX2. The quantification was performed by calculating the average percentage of nestin + /SOX2 + cells out of the total cells in three wells. Quantification was carried out with cells cultured in monolayer

Article Snippet: The following primary antibodies were used: nestin (1:400; sc-33,677; Santa Cruz Biotechnology, Dallas, TX, USA) and SOX2 (1:400; bs-23176R-A488; Bioss, Massachusetts, USA) (neural progenitor markers); GFAP (1:400; sc-33,673; Santa Cruz Biotechnology) (glial marker); MBP (1:400; bs-0380R-A488; Bioss) and β3-tubulin (1:400; sc-80,005; Santa Cruz Biotechnology) (neuronal markers).

Techniques: Immunocytochemistry, Cell Culture, Staining, Marker

Journal: BMC Veterinary Research

Article Title: Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells

doi: 10.1186/s12917-023-03757-3

Figure Lengend Snippet: Primer sequences used for real-time quantitative PCR

Article Snippet: The following primary antibodies were used: nestin (1:400; sc-33,677; Santa Cruz Biotechnology, Dallas, TX, USA) and SOX2 (1:400; bs-23176R-A488; Bioss, Massachusetts, USA) (neural progenitor markers); GFAP (1:400; sc-33,673; Santa Cruz Biotechnology) (glial marker); MBP (1:400; bs-0380R-A488; Bioss) and β3-tubulin (1:400; sc-80,005; Santa Cruz Biotechnology) (neuronal markers).

Techniques: Sequencing

Journal: Biomedicines

Article Title: Morphological and Immunocytochemical Characterization of Paclitaxel-Induced Microcells in Sk-Mel-28 Melanoma Cells

doi: 10.3390/biomedicines12071576

Figure Lengend Snippet: Primary and secondary antibodies.

Article Snippet: Caspase-6 , Primary antibody: rabbit anti-human, rabbit polyclonal , ALEXA FLUOR ® 555 conjugated Ex.553 nm/Em.568 nm , 1:100 , BS-0151R-A555, Bioss antibodies/Nordic BioSite, Täby, Sweden.

Techniques: Concentration Assay, Recombinant

Journal: Nanomaterials

Article Title: Inhibition of TRPV1 Channel Activity in Human CD4 + T Cells by Nanodiamond and Nanoplatinum Liquid, DPV576

doi: 10.3390/nano8100770

Figure Lengend Snippet: ( A – D ) DPV576 does not modulate the expression of TRPV4 on CD4 + T cells. Inactivated and anti-CD3/CD28 activated CD4 + T lymphocytes were exposed to DPV576 for 24 h. The cells were stained for TRPV channels. Expression of TRPV4 on non-activated T cells ( A , B ). Anti-CD3/CD28 activated T cells ( C , D ). Data is representative of three experiments. Blue: Without DPV576; Red: DPV576 exposed lymphocytes; Green: Isotype.

Article Snippet: Briefly, cells were collected and centrifuged, re-suspended in PBS (phosphate buffered saline) 2% FBS, and incubated with specific antibodies for TRPV1-AL647 (bs-1931R-A647) or TRPV4-Alexa 488 (bs-6425R-A488) (Bioss Inc., Woburn, MA, USA) at a dilution of 1:100 for 1 h. Subsequently, the cells were washed and a minimum of 10,000 cells were acquired on FACS Calibur (Becton Dickinson, San Jose, CA, USA).

Techniques: Expressing, Staining